The Challenge starts near the western boundary of iMfolozi Game Reserve just south of Cengeni gate in the Mbhuzane section of the park. The route heads south along a management jeep track, which is not open to tourist vehicles. The first few kilometres are mainly downhill towards the White IMfolozi River with some patches of soft sand as you get closer to the river.
Before the halfway mark the route starts climbing fairly sharply to the top of Ndleke. Enjoy the view while you can, because the descent down Mduba will require all your concentration. There are some steep sections with lots of loose rocks. From here the route rolls to iSiyembeni, where the White and Black iMfolozi Rivers join.
You cross both rivers and ride into the Masinda section. At Mambeni you join a tar road for the final couple of kilometres to the finish near the Game Capture complex and Centenary Centre.